Stable transfection of Eimeria intestinalis and investigation of its life cycle, reproduction and immunogenicity

Rabbit coccidiosis, caused by infection of Eimeria spp.is one of the most severe parasitic diseases in rabbits.E.intestinalis is one of the most immunogenic species in rabbit coccidia.Due to the lack of genomic information and unsuccessful in vitro cultivation, genetic manipulation of rabbit coccidia lagged behind other apicomplexan parasites.Using

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Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis.The sequence analysis revealed the ilootpaperie expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400.The proteins exhibit a 70-80% homology with o

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The Comic Latin Grammar in Victorian England

This paper presents the first scholarly analysis of The Comic Latin Grammar by Percival Leigh, a satirical textbook of Latin grammar published in London in 1840.Sections I and II analyze the role of Latin education and the rapid publication of Latin grammar books during the nineteenth century.Sections III pet calming peanut butter and IV conduct cl

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Second illness due to subsequent infection in a domestic cat

Case summary A castrated male domestic shorthair cat from a wooded area in Missouri had recovered from typical severe cytauxzoonosis at 4 years of age, after intensive in-hospital supportive care and administration of atovaquone and azithromycin.At 11 years of age, the same cat again experienced an acute febrile illness compatible with cytauxzoonos

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